The three-year-old was filmed sitting on her dad's lap during Monday's show.
The "Weekend Update" bit had starring Chloe Fineman as JoJo declaring "I'm the first gay girl in the world!"
The country icon also sang her sitcom theme "I'm a Survivor" with Jennifer Hudson on Thursday (May 2) and talked about being the "new kid" on "The Voice" last season.
She also performed "Acknowledge Me" on Wednesday night's (May 1) "Tonight Show."
She also told Jennifer Hudson that she once turned down Elvis because she was "nervous of his reputation."
The Hot 100 No. 1 single spent eight weeks at the top of the charts back in 2014.
"See you next time, happy blending!" read the message from singer/guitarist Trey Anastasio.
The trio will play one of the late singer's favorite songs by gospel group Mary Mary.
Hazbin Hotel is unique among the longest-running No. 1 soundtracks for two reasons.
Monday night's (April 22) show found Maluma bonding with Venezuelan singer Mafe.
