Dave Grohl, Kylie Minogue, Ed Sheeran, Bruce Springsteen, Sting and many others appear in the new doc.
The show is set to premiere on HBO on June 4.
The musical film also features Ciara, Danielle Brooks and Taraji P. Henson.
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"This is an invitation from me, Katy Perry," the "Roar" singer pleaded.
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The non-binary artist cautioned about putting musicians and other celebrities on a pedestal.
"It really breaks my heart that there are young people growing up in a world that doesn't protect them," the star said on stage at the Chi Health Center in Omaha.
The singer wore a blonde wig and prosthetic breasts during the performance.
The upcoming Searchlight Pictures film stars Timothée Chalamet as Dylan.
